Horizon wide-format objective lens installed at customer site – Allen Institute for Brain Science. Version shown is for the JEOL 1200 platform. Photo credit: Jenny Burns, Allen Institute.
100mm multi orifice wafer. Image credit: Kim Gruver, Allen Institute for Brain Science.
5mm x 5mm fast image montage (100 x 100 tiles) from a 40nm thick coronal section of mouse brain (hemisphere) recorded using a JEOL-1200EXII at 750x magnification. Image credit: Kim Gruver, Allen Institute for Brain Science.
Ultra-wide Tissue Mapping
A standard 3mm TEM grid may have around 2mm of usable x-y travel. This leaves small mammal (e.g. mouse) brain imaging beyond reach. The Horizon Wide Format Objective Lens combined with wafer-size sample carriers, enables suspended tissue sections with widths of at least 10mm to be imaged. Each wafer can hold from several tens of whole hemispheres to several hundred smaller ribbon-cut sections.
Luxel® Membrane Technology
At turboTEM, we have partnered with Luxel to develop our GridDisc wafer sample carriers. Luxel’s LUXFilm® Polyimide membrane is 5X stronger than Formvar and allows for support film membranes exceeding 10mm in diameter. The LUXFilm® Polyimide TEM supports are thin (50nm), uniform and robust against handling, beam exposure and charging-free.
High Resolution Detail
The Horizon Wide Format Objective Lens maintains and expands on the original column performance. Fine details with high contrast are resolved, facilitating accurate segmentation and data reconstruction.
Tests at higher magnification with 4.5-nanometre pixel size, show fine synaptic-resolution detail with minimal distortion.
Column Compatible
The Horizon Wide Format Objective Lens is compatible with the original JEOL aperture strip, enabling optimum contrast for TEM imaging. The original vacuum system and logic of the host column are preserved, with extra pumps and valves added to meet the customer specification for vacuum level or load-lock sample exchange configurations.
(right) a high magnification image from a 40nm thick section of mouse brain (hemisphere) recorded using a JEOL-1200EXII at 3000x magnification. Image credit: Kim Gruver, Allen Institute for Brain Science.
Designed & Made in Ireland
The Horizon Wide Format Objective Lens is designed and manufactured here in Ireland using the highest quality materials. Every objective lens is laser engraved with a serial number to trace it through manufacturing and quality control and out into the field for installation. After numbering, each unit is dimensionally inspected and shipped with a report certificate.
Frequently Asked Questions (FAQ)
What models of microscopes is Horizon available for?
The Horizon Wide format objective lens is currently available for the JEOL 1200 platform. We plan to bring this to other columns based on demand.
How long does it take to install the Horizon Wide format objective lens?
The Horizon wide-format objective lens requires a column-split, and can be installed by two people in less than a week.
How easy is it to align the microscope after replacing the objective lens?
The Horizon wide-format objective lens operates at the same beam voltage as the stock microscope, and preserves the original pole-piece dimensions. This means that the user can align the microscope starting from the last saved settings even with the new lens.
Can the port configuration on the pole-piece be changed for other uses?
Yes! The Horizon wide-format objective lens is a fabricated by turboTem in Ireland. This means we can add/remove/customise many of the ports to a specific user need. Examples might include a tilting stage, spectroscopy ports for EDX or CL, or access for in-situ gas or mechanical feedthroughs.